《植物生理学报》 2013, 49(7): 700-708

C₄植物白花菜叶片发育不同阶段的基因表达谱分析

刘征^1,*, 杨尚君¹, 张书敏¹, 赵彦宏², 高双成³, 韩渊怀⁴

¹河北大学生命科学学院, 河北保定071002; ²鲁东大学农学院, 山东烟台264025; ³河南科技大学农学院, 河南洛阳471003; ⁴山西农业大学农学院, 山西太谷030801

通信作者：刘征；E-mail: liuzhengxp@yeah.net；Tel: 0312-5079364

摘　要：

C₄植物白花菜与拟南芥是姊妹科的近缘植物, 是一种新近开发的研究C4光合作用的模式植物。对其叶片发育不同阶段进行了基因表达谱的比较和分析, 结果表明在叶脉正在发育的幼叶中, 细胞分裂旺盛, 蛋白质大量合成, 表现为核糖体蛋白基因、组蛋白基因、翻译因子基因、分子伴侣基因等的表达上调; 幼叶光合作用相关基因表达下调, 暗示着光合器官仍处于发育阶段; 多种激素相关的基因如生长素、脱落酸、赤霉素、油菜素内酯、细胞分裂素、茉莉酸合成途径基因或信号传导基因参与了叶片的发育, 表明激素对发育的调控作用。

关键词：白花菜; 基因表达谱; 叶片发育; 蛋白质合成; 光合作用; 植物激素

收稿：2013-04-18   修定：2013-05-15

资助：教育部留学回国人员科研启动基金(教外司留2011-1139)、河北大学引进人才项目(2010-185)和国家自然科学基金(30800682和31201199)。致谢 英国剑桥大学植物科学系Julian Hibberd博士对本研究提供了重要的指导和支持。

Gene Expression Profiling during Leaf Development of a C₄ Plant, Cleome gynandra

LIU Zheng^1,*, YANG Shang-Jun¹, ZHANG Shu-Min¹, ZHAO Yan-Hong², GAO Shuang-Cheng³, HAN Yuan-Huai⁴

¹College of Life Sciences, Hebei University, Baoding, Hebei 071002, China; ²School of Agriculture, Ludong University, Yantai, Shandong 264025, China; ³College of Agronomy, Henan University of Science and Technology, Luoyang, Henan 471003, China; ⁴College of Agriculture, Shanxi Agricultural University, Taigu, Shanxi 030801, China

Corresponding author: LIU Zheng; E-mail: liuzhengxp@yeah.net; Tel: 0312-5079364

Abstract:

Cleome gynandra is a C₄ species closely related with Arabidopsis thaliana and a newly developed non-sequenced model plant for C₄ photosynthesis. Here we conducted the comparative gene expression profil-ing to study the leaf development of C. gynandra. Data analysis showed in young leaves when veins were de-veloping, expressions of ribosomal protein genes, histone genes, translational factors genes, molecular chaper-onin genes, etc were up-regulated, indicating that cells were dividing quickly and a lot of nascent proteins were being produced. Photosynthesis related genes were down-regulated in young leaves, suggesting that photosyn-thetic apparatuses were still stay in developing stage. Various genes related to phytohormones such as auxin, abscisic acid, gibberellin, brassinosteroid, cytokinin, jasmonic acid were participating in the leaf development, showing their regulating functions on development.

Key words: Cleome gynandra; gene expression profiling; leaf development; protein synthesis; photosynthesis; phytohormones